Coding

Part:BBa_K5122003:Design

Designed by: Thi Huyen Mai Nguyen   Group: iGEM24_Stockholm   (2024-09-27)


RBS -> guaA -> RBS -> guaB -> RBS -> aeBlue


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 252
    Illegal AgeI site found at 1057
    Illegal AgeI site found at 2013
    Illegal AgeI site found at 2601
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2301


Design Notes

The aeBlue marker was added as a transformation marker. The guaA and guaB were put on the same insert to simplify transfection.



Source

guaA and guaB: coding sequences from Escherichia coli str. K-12 substr. MG1655 genome (NCBI). aeBlue: The protein was first extracted and characterized by Shkrob et. al. 2005 under the name aeCP597. This version is codon optimized for E coli by Bioneer Corp.


References